EBTT 2007
Archived edition

EBTT 2007

Electroporation based Technologies and Treatments — International Scientific Workshop & Postgraduate Course

11–17 November 2007Ljubljana, SloveniaECTS credits

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

Whether you are a postgraduate student or you just want to learn and contribute to the discussion, you were kindly invited to attend the workshop and the course on electroporation technologies and treatments. Abstract submission was strongly encouraged and was necessary if credits were sought.

Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana.

Organising committee

Chair: Peter Kramar
Members: Alenka Maček Lebar, Maša Kandušer, Tadej Kotnik, Gorazd Pucihar, Mojca Pavlin, Nataša Pavšelj, Anže Županič

Course content

Key lectures & topics

Cells and tissues in electric fields

D. Miklavčič — biological cell & plasma membrane; resting & induced transmembrane voltage; static & dynamic fields; temperature & conductivity changes.

Physical chemistry of membrane electroporation

E. Neumann — electrochemical membrane thermodynamics; electroporative transport.

Electroporation in vitro

J. Teissié — cells in suspension and in 2D tissue models; parameters governing efficiency in vitro.

Electroporation in vivo

L. M. Mir — electroporation of cells in tissue; methods for detecting cell electropermeabilization in vivo.

Development of devices and electrodes

D. Miklavčič — devices for in vitro, in vivo and clinical purposes; electrodes.

Electrochemotherapy of tumours

G. Serša — experiments in vitro & in vivo; current clinical applications; future perspectives.

Gene transfection

L. M. Mir & J. Teissié — gene transfection in vitro/in vivo; gene expression regulation by siRNA electrotransfer.

Electroporation in biotechnology

J. Teissié — water sterilization and other applications; future perspectives.

Transdermal drug delivery

V. Préat — transdermal delivery using electroporation; intradermal & topical delivery of DNA.

Students & participants presentations

Short presentations by students and participants.

Additional topics

Faculty

Co-directors, faculty & invited lecturers

Co-DirectorDamijan Miklavčič
Co-DirectorLluis M. Mir

Faculty members & lecturers

  • Damijan MiklavčičUniversity of Ljubljana, Slovenia
  • Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
  • Eberhard NeumannUniversity of Bielefeld, Germany
  • Véronique PréatCatholic University of Louvain, Brussels, Belgium
  • Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
  • Justin TeissiéCNRS & IPBS, Toulouse, France

Invited lecturers

  • Bettina CoudercInstitut Claudius Regaud, Toulouse, France
  • Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
  • Rumiana DimovaMax Planck Institute of Colloids & Interfaces, Germany
  • Muriel GolzioCNRS & IPBS, Toulouse, France
  • Sergej KakorinUniversity of Bielefeld, Germany
  • Tadej KotnikUniversity of Ljubljana, Slovenia
  • Marie-Pierre RolsCNRS & IPBS, Toulouse, France
  • Richard HellerUniversity of South Florida, USA
  • Youssef TamzaliVeterinary School of Toulouse, France
  • Tomaž JarmUniversity of Ljubljana, Slovenia
  • P. Thomas VernierUniversity of Southern California, USA

Programme & schedule

Day-by-day schedule

11–17 November 2007. Download the full schedule (PDF) · Preliminary schedule (PDF)

Sunday 11 November — Arrival

18:30Registration: Hotel Slon
19:00Welcome drink

Day 1 — Monday 12 November

8:30Opening lecture
Damijan Miklavčič
9:15Cells in electric fields
Tadej Kotnik
10:00Coffee break
10:30Electric properties of tissues and their changes during electroporation
Damijan Miklavčič, Nataša Pavšelj
12:00Lunch break
13:30Effects of ultra short electric pulses
P. Thomas Vernier
14:30Coffee break
15:00Students' presentations
15:45Practical work and discussion groups (1)

Day 2 — Tuesday 13 November

8:30Electrochemistry of cell membranes
Eberhard Neumann
9:15Dynamics of coupled flow processes
Eberhard Neumann
10:00Coffee break
10:30Electropermeabilization in vitro
Justin Teissié
12:00Lunch break
13:30Electroporation of lipid vesicles
Rumiana Dimova
14:30Coffee break
15:00Students' presentations
15:45Practical work and discussion groups (2)

Day 3 — Wednesday 14 November

8:30Development of devices and electrodes
Damijan Miklavčič
10:00Coffee break
10:30Transduction, transcription, regulation and gene expression
Bettina Couderc
11:15Lunch break
12:00Social event

Day 4 — Thursday 15 November

8:30Electropermeabilization in vivo
Lluis M. Mir
9:15Electroporation in electrochemotherapy of tumours
Gregor Serša
10:00Coffee break
10:30Preclinical and clinical electrochemotherapy
Gregor Serša
11:15Blood flow measuring methods
Tomaž Jarm
12:00Lunch break
13:30Electrochemotherapy in veterinary medicine
Youssef Tamzali, Nataša Tozon, Maja Čemažar
14:30Coffee break
15:00Students' presentations
15:45Practical work and discussion groups (3)

Day 5 — Friday 16 November

8:30Skin and transdermal drug delivery
Véronique Préat
9:15Gene transfection in vitro
Marie-Pierre Rols
10:00Coffee break
10:30Gene transfection in vivo
Lluis M. Mir
11:15Gene expression regulation by siRNA electrotransfer
Muriel Golzio
12:00Lunch break
13:30Gene Delivery with Electroporation: Translation to the clinic
Richard Heller
14:30Coffee break
15:00Students' presentations
15:45Practical work and discussion groups (4)

Day 6 — Saturday 17 November

8:30Electroporation in transdermal drug delivery
Véronique Préat
9:15Electroporation in skin gene delivery
Véronique Préat
10:00Coffee break
10:30Application of electroporation in biotechnology
Justin Teissié
11:15Closing lecture: summary and perspectives
Lluis M. Mir
12:00Lunch break
13:30Exams
15:00Coffee break
15:30Certificates and goodbye

See also: Practical work (PDF) · Instructions (PDF)

Registration

Tuition fee & deadlines

The tuition fee (580 EUR) included attendance to the lectures, book of abstracts and course material, lunches and social events, certificate of attendance and examination (ECTS credits). A limited number of grants for student accommodation was available on request.

Important deadlines (archived):
Registration & abstract submission: 1 September 2007.
Payment of the tuition fee: 10 October 2007.

Forms

Abstracts & proceedings

Abstract submission & proceedings

Download the abstract submission form and instructions for authors. For abstract writing, the enclosed MS Word template was used.

EBTT 2007 proceedings cover

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Getting here & staying

Venue & accommodation

Held at the Faculty of Electrical Engineering, University of Ljubljana. Map of Ljubljana centre

Support

Sponsors & partners

IGEA Inovio PlasmidFactory BetaTech Zeiss Clinigene

Supported also by the Bioelectrochemical Society, the Slovenian Research Agency (ARRS), the French–Slovenian Scientific Cooperation (PROTEUS Programme) and CNRS.

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros
nina@albatros-bled.com
tel +386 4 5780 351 · fax +386 4 5780 355

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