November 11-17, 2007

Ljubljana, Slovenia

Electroporation based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

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Key lectures and content of the course:

Cells and tissues in electric fields

D. Miklavèiè
  • Biological cell and its plasma membrane
    		•
  • Resting and induced transmembrane voltage
    		•
  • Static and dynamic electric fields in biological tissues
    		•
  • Temperature and conductivity changes in tissues
    		•

    Physical Chemistry of Membrane Electroporation

    E. Neumann
  • Electrochemical membrane thermodynamics
    		•
  • Electroporative transport
    		•

    Electroporation in vitro

    J. Teissié
  • Electroporation of cells in suspension
    		•
  • Electroporation of cells in 2D tissue model
    		•
  • Parameters that determine the efficiency of electroporation in vitro
    		•

    Electroporation in vivo

    L.M. Mir
  • Electroporation of cells in tissue
    		•
  • Methods for detecting cell electropermeabilization in vivo
    		•

    Development of devices and electrodes

    D. Miklavèiè
  • Devices for in vitro and in vivo experimental work
    		•
  • Devices for clinical purposes
    		•
  • Electrodes for in vitro and in vivo
    		•

    Application of electroporation in electrochemotherapy of tumors

    G. Sersa
  • Experiments in vitro
    		•
  • Experiments in vivo
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  • Current clinical applications
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  • Future perspectives
    		•

    Application of electroporation in gene transfection

    L.M. Mir,
  • Gene transfection in vitro
    		•

    J. Teissié
  • Gene transfection in vivo
    		•
  • Gene expression regulation by siRNA electrotransfer
    		•
  • Future perspectives
    		•

    Application of electroporation in biotechnology

    J. Teissié
  • Water sterilization
    		•
  • Other applications
    		•
  • Future perspectives
    		•

    Electroporation in transdermal drug delivery

    V. Preat
  • Transdermal drug delivery using electroporation
    		•
  • Intradermal and topical delivery of DNA by means of electroporation
    		•

    Additional topics:
  • Regulation of gene expression
    		•

  • Gene Delivery with Electroporation: Translation to the Clinic
    		•

  • Electrochemotherapy in veterinary medicine
    		•

  • Blood flow measuring methods
    		•

  • Ultra-Short Electric Pulses
    		•

    Students and participants short presentation